Polymerase Chain Reaction (PCR) enables DNA amplification that is an essential part of genotyping. Through genotyping, DNA sequence variations in an individual can be determined, and genetic disorders or diseases are identified. PCR’s ability to target specific segments in genetic material means that PCR testing can be used to identify even trace segments of targeted sequences in DNA or RNA fragments.
Protecting Samples During PCR
PCR is extremely sensitive and susceptible to contamination. Particularly when used in applications like in vitro fertilization, available genetic samples can be limited. If the cells collected for pre-implantation genetic testing (PGT) are contaminated, it could result in an unsuccessful embryo transfer or a child affected by a genetic disease. Thermocyclers, or PCR machines, should be operated in a biocontainment cabinet (PCR hood) to help prevent cross-contamination that can adversely affect samples.
Genotyping can help provide priceless information. Particularly as IVF becomes a more common choice for families trying to conceive. It is therefore essential to safeguard samples from cross-contamination. In addition to following good laboratory practices, PCR machines (or thermocyclers) should be used in a laminar flow hood designed to protect materials on the work surface.
The Air Science® PCR laminar flow cabinet employs Air Science Multiplex™ HEPA filtration technology to create a safe, energy-efficient contaminant-free environment. Purair PCR cabinets are equipped with a germicidal UV lamp for safe, effective decontamination between PCR amplification. The microbial powder finish coating further helps prevent contamination. Laminar airflow protects equipment and other work zone contents from particulates for applications sensitive to cross-contamination.