Polymerase chain reaction (PCR) is a simple and inexpensive technique to make multiple copies of a targeted nucleotide sequence from a DNA sample and to amplify sequences from small samples. This technique is widely used in genetics laboratories that work with DNA and RNA.
Because PCR amplification is extremely sensitive to contamination, prevention of contamination requires good laboratory practices to minimize external or cross-contamination during reagent preparation, sample preparation and sample amplification.
The Purair® PCR laminar flow cabinet employs the Air Science® Multiplex™ HEPA filtration technology to create a safe, energy-efficient contaminant-free environment. This PCR workstation is ideally suited for use with non-hazardous contaminants and when flexible access to the instrumentation in the cabinet is required.
- Provides sterile work zone for aseptic techniques.
- Air cleanliness meets and exceeds ISO Class 5.
- Energy saving LED lighting.
- Vertical laminar airflow with HEPA filtration.
- Equipped with germicidal UV lamp for decontamination.
- 360 degree visibility with UV absorbing polycarbonate construction.
- Purair PCR cabinets are available in three model sizes with various options.
- Designed for desktop use or installation on an optional base stand or mobile cart.
A. Control Panel: The integrated control panel features an On/Off switch for the fan, light, and UV lamp timer.
B. Main Filter: Main HEPA filter with 99.99% efficiency for 0.3 micron particulates.
C. Pre-Filter: The pre-filter can be changed while the unit is operating to prevent operator exposure to chemical vapors.
D. Fan: High-performance ebm-papst EC fan.
E. Stand: Optional base stand converts to mobile cart with optional locking casters.
F. Lighting: Compact LED cabinet lamp located away from laminar flow area.
G. UV Lamp: Built-in ultraviolet lamp with timer permits decontamination between PCR cycles. Includes a double-flap safety cover of 0.2" (5 mm) UV absorbing 254 nm beta radiation resistant polycarbonate; provides superior operator protection while allowing easy access to the work zone. Timer can be set over range of 0 to 60 minutes or set to HOLD.
H. Pass Through Ports: Convenient rear wall pass-through ports for safe routing of instrument cords, cables, and leads.
I. Filter Door Lock: Prevents unauthorized removal or accidental exposure to dirty filters.
J. Ergonomic Design: Ergonomically angled front improves reach and user comfort.
K. Work Surface: Large polypropylene work surface, white or black standard with optional stainless steel work surface available.
L. Decontamination Shelf: Mounted on the back wall near the UV lamp for maximum exposure.
M. Magnetic Door Latch: Safety interlock prevents operator exposure to UVs when door is opened during decontamination cycle.
N. Double-Hinged Self-Locking Front Sash: The magnetic sash closure protects samples on the work surface from contamination with up to 90 fpm airflow.
360 Degree Visibility: Clear back and side panels
allow ambient illumination into the chamber and provide users with an unobstructed view of its contents.
Standards Compliant: Performance specifications and construction meet or exceed OSHA, ANSI and relevant international standards to assure operator safety.
Construction: All models are available in either metal or polypropylene construction, specify metal or polypropylene when ordering. Available in 120V, 60Hz or 220V, 50Hz models.
Steel Support Frame: The chemical resistant epoxy coated steel frame adds mechanical strength. Optional all polypropylene construction is available if desired.