Polymerase chain reaction (PCR) is a simple and inexpensive technique to make multiple copies of a targeted nucleotide sequence from a DNA sample, and to amplify sequences from small samples. This technique is widely used in genetics laboratories that work with DNA and RNA.
Because PCR amplification is extremely sensitive to contamination, prevention of contamination requires good laboratory practices to minimize external or cross-contamination during reagent preparation, sample preparation and sample amplification.
The Purair® PCR laminar flow cabinet employs the Air Science® Multiplex™ HEPA filtration technology to create a safe, energy-efficient contaminant-free environment. It is ideally suited for use with non-hazardous contaminants and when flexible access to the instrumentation in the cabinet is required.
Convenience and Construction Benefits
- Purair PCR cabinets are available in three model sizes with various options.
- Cabinets are shipped fully assembled with standard power cords; no installation required.
- The integral UV lamp fixtures are pre-installed.
- All filters are pre-installed, easy to access and easy to change.
- Products are designed for desktop use, or may be installed on an optional base stand or mobile cart.
- The high capacity air handling system delivers flow velocity of 0.45 m/s or 90 fpm.
- Purair PCR cabinets are constructed of steel with a microbial powder finish coating.
- The optional stainless steel work surface includes lips on all four sides to contain spills.
A. Task Lighting: Fluorescent cabinet lamp located away from laminar flow area.
B. Ultraviolet Lighting: Built-in ultraviolet lamp with timer permits decontamination between PCR cycles. Includes a double-flap safety cover of 0.2" (5 mm) UV absorbing 254 nm beta radiation resistant polycarbonate; provides superior operator protection while allowing easy access to the work zone. Timer can be set over range of 0 to 60 minutes or set to HOLD.
D. Decontamination Shelf: Mounted on the back wall is proximate to the UV lamp for maximum exposure.
E. Filter: Camfil Farr main HEPA filter with 99.99% efficiency for 0.3 micron particulates. The pre-filter can be changed while the unit is operating to prevent operator exposure to chemical vapors.
F. Pass Through Ports: Convenient rear-wall pass through ports for safe routing of instrument cords, cables and leads.
G. Double-Hinged Self-Locking Front Sash: With overlap closed, the cabinet sash protects samples on the work surface from contamination with 100 fpm airflow. The sash completely closes unit off when not in use and is easy to open and latch.
H. Fan: High-performance ebmpapst™ centrifugal fan.
I. Stand: Optional base stand converts to mobile cart with optional locking casters.
J. Work Surface: Large polypropylene work surface, white or black standard, with optional stainless steel work surface available.
K. Filter Door Key: Prevents unauthorized removal or accidental exposure to dirty filters.
L. Magnetic Door Latch: Safety interlock prevents accidental operator exposure when door is opened.
360 Degree Visibility: Clear back and side panels
allow ambient illumination into the chamber and provide users with an unobstructed view of its contents.
Standards Compliant: Performance specifications and construction meet or exceed OSHA, ANSI and relevant international standards to assure operator safety.
Construction: All models are available in either metal or polypropylene construction, specify metal or polypropylene when ordering. Available in 120V, 60Hz or 220V, 50Hz models.
Steel Support Frame: The chemical resistant epoxy coated steel frame adds mechanical strength. Optional all polypropylene construction is available if desired.