Polymerase chain reaction1 (PCR) is a simple and inexpensive technique to make multiple copies of a targeted nucleotide sequence from a DNA sample, and to amplify sequences from small samples. This technique is widely used in genetics laboratories that work with DNA and RNA.
Because PCR amplification is extremely sensitive to contamination, prevention of contamination requires good laboratory practices to minimize external or cross-contamination during reagent preparation, sample preparation and
The Purair PCR laminar flow cabinet employs the Air Science Multiplex™ HEPA filtration technology to create a safe, energy-efficient contaminant-free environment. It is ideally suited for use with non-hazardous contaminants and when flexible access to the instrumentation in the cabinet is required.
1 Polymerase chain reaction (PCR) is a patented process owned by Hoffman La Roche.
These cabinets are intended for use in nonhazardous applications where biological or biohazard byproducts are not generated and user protection is not required.
A. Task Lighting: Fluorescent cabinet lamp located away from laminar flow area.
B. Ultraviolet Lighting: Built-in ultraviolet lamp with timer permits decontamination between PCR cycles. Includes a double-flap safety cover of 0.2" (5 mm) UV absorbing 254 nm beta radiation resistant polycarbonate; provides superior operator protection while allowing easy access to the work zone. Timer can be set over range of 0 to 60 minutes or set to HOLD.
C. Comfort: Ergonomically angled front improves reach and user comfort.
D. Decontamination Shelf: Mounted on the back wall is proximate to the UV lamp for maximum exposure.
E. Filter: Camfil Farr main HEPA filter with 99.99% efficiency for 0.3 micron particulates. The pre-filter can be changed while the unit is operating to prevent operator exposure to chemical vapors.
F. Pass Through Ports: Convenient rear-wall pass through ports for safe routing of instrument cords, cables and leads.
G. Double-Hinged Self-Locking Front Sash: With overlap closed, the cabinet sash protects the operator with 100 fpm airflow. The sash is easy to open and latch.
H. Fan: High-performance ebmpapst™ centrifugal fan.
I. Stand: Optional base stand converts to mobile cart with optional locking casters.
J. Work Surface: Large polypropylene work surface, white or black standard, with optional stainless steel work surface available.
K. Filter Door Key: Prevents unauthorized removal or accidental exposure to dirty filters.
L. Magnetic Door Latch: Safety interlock prevents accidental operator exposure when door is opened.
360 Degree Visibility: Clear back and side panels
allow ambient illumination into the chamber and provide users with an unobstructed view of its contents.
Standards Compliant: Performance specifications and construction meet or exceed OSHA, ANSI and relevant international standards to assure operator safety.
Construction: All models are available in either metal or polypropylene construction, specify metal or polypropylene when ordering. Available in 120V, 60Hz or 220V, 50Hz models.
Steel Support Frame: The chemical resistant epoxy coated steel frame adds mechanical strength. Optional all polypropylene construction is available if desired.
The integrated control panel features an On/Off switch for the fan, light and UV lamp timer.
Performance specifications and construction meet or exceed OSHA, ANSI and relevant international standards to assure operator safety.
The Air Science® Performance Advantage
Each Air Science fume hood includes features expressed through sound design and certified quality construction. Options and accessories add functional performance to meet specific applications.
PURAIR® PCR AIRFLOW PATTERN
Purair® PCR-36 shown with Multiplex™ filtration system.
Room air enters from the top of the cabinet through the disposable pre-filter where larger particles are trapped, increasing the service life of the main HEPA filter.
Air is forced evenly across the HEPA filter to deliver a flow of pure, uniform air within the work zone to dilute and flush airborne contaminants from the work area.
A nominal filter face velocity of 0.45 m/s (90 fpm) ensures that there is sufficient number of air changes within the work zone to eliminate cross-contamination and to maintain optimum cleanliness.
Purified air travels across the work zone to the work surface in a vertical, unidirectional downflow stream, and then exits the work zone across the open cabinet front area of the cabinet.
A. The main filter is easy to replace; no tools required. The filter clamps tightly against the filter gasket to prevent filter bypass and to maintain filter integrity.
Air Science uses long-life Camfil Farr HEPA filters without aluminum separators to increase filter efficiency, minimize the potential for leakage and increase filter life.
Energy-Saving Blower System
Purair PCR cabinets incorporate energy-efficient ebmpapst™ permanently lubricated direct drive centrifugal blowers for maximum operational savings, low noise and minimal vibration.
Purair® PCR cabinets maintain a 0.45 m/s or 90 fpm air flow velocity, measured 6”/150mm from the filter face, with a uniformity of +/- 20% across the filter face. This face velocity is in compliance with USA and international standards for safety and performance.
Convenience and Construction Benefits
Specifications are subject to change without notice.
*Average airflow measured 6"/150mm from filter face. Uniformity is +/- 20%.
**Camfil Farr filters; HEPA efficiency: 99.99% at 0.3μm
Options and Accessories
The optional Monitair microprocessor controller monitors and displays cabinet operating parameters, airflow, containment, and filter condition; emits audio and visual alerts if conditions become unsafe, all on a LCD display.
Purair® PCR cabinets maintain a 0.45 m/s (90 fpm) airflow velocity, measured 6" (150 mm) from the filter face, with a uniformity of +/- 20% across the filter face. This face velocity is in compliance with U.S.A. and international standards for safety and performance. The HEPA filters are easy to replace with common tools.
Standards & Compliance
Air Science® 120 6th Street Fort Myers, FL 33907
T/ 239.489.0024 Toll Free/800.306.0656 F/800.306.0677